All distances are from Gainesville city limit to destination city limit, except where marked with an asterisk.
A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | Y | Z
| A | |
| Altamonte Springs | 103 |
| Amelia Island | 98 |
| Anthony | 28 |
| Apalachicola | 197 |
| Apopka | 94 |
| Arcadia | 184 |
| Auburndale | 186 |
| Avon Park | 156 |
| D | |
| Dade City | 95 |
| Day | 81 |
| Daytona Beach | 98 |
| Deerfield Beach/td> | 298 |
| Defuniak Springs | 263 |
| Deland | 98 |
| Delray Beach | 287 |
| Deltona | 110 |
| Destin | 283 |
| Disney World | 121 |
| Dowling Park | 85 |
| Dunedin | 127 |
| Dunnellon | 50 |
| E | |
| Eglin AFB | 303 |
| Eustis | 82 |
| F | |
| Fanning Springs | 38 |
| Fernandina Beach | 100 |
| Flagler Beach | 82 |
| Fort Lauderdale | 312 |
| Fort McCoy | 35 |
| Fort Myers | 231 |
| Fort Pierce | 217 |
| Fort Walton Beach | 289 |
| Fort White | 34 |
| Frost Proof | 158 |
| G | |
| Green Cove Springs | 54 |
| Grenelefe | 134 |
| Gulfport | 145 |
| H | |
| Haines City | 124 |
| Hallandale | 322 |
| Hastings | 65 |
| Hialeah | 326 |
| Hilliard | 87 |
| Hobe Sound | 259 |
| Hollywood | 319 |
| Homestead | 346 |
| Homosassa | 67 |
| Horseshoe Beach | 69 |
| Howey-in-the-Hills | 90 |
| Hudson | 101 |
| I | |
| Immokalee | 250 |
| Indialantic | 177 |
| Indiantown | 244 |
| Inglis | 54 |
| Innisbrook | 115 |
| Interlachen | 27 |
| Interlachen (from Andrews Center)* | 29 |
| Inverness | 62 |
| Islamorada | 409 |
| J | |
| Jacksonville | 69 |
| Jacksonville Airport | 89 |
| Jacksonville Beach | 85 |
| Jasper | 75 |
| Jennings | 87 |
| Jupiter | 255 |
| K | |
| Key Biscayne | 347 |
| Key Largo | 375 |
| Key West | 474 |
| Keystone Heights | 24 |
| Keystone Heights (from Andrews Center)* | 13 |
| Keystone Heights (from NW Campus)* | 34 |
| Kissimmee | 123 |
| N | |
| Naples | 264 |
| Navarre Beach | 314 |
| New Port Richie | 108 |
| New Smyrna | 111 |
| New Smyrna Beach | 112 |
| Niceville | 280 |
| Nokomis | 195 |
| North Miami | 327 |
| North Miami Beach | 327 |
| O | |
| Ocala | 37 |
| Ocoee | 99 |
| Okeechobee | 216 |
| Oklawaha | 56 |
| Old Town | 41 |
| Oleno State Park | 49 |
| Ona | 168 |
| Orange Heights | 15 |
| Orange Lake | 22 |
| Orange Park | 61 |
| Orange Springs | 32 |
| Orlando | 109 |
| Orlando International Airport | 128 |
| Ormond Beach | 93 |
| Q | |
| Quincy | 167 |
| R | |
| Raiford | 38 |
| Raiford (from Andrews Center)* | 14 |
| Reddick (from NW Campus)* | 31 |
| Redington Beach | 153 |
| Riviera Beach | 262 |
| Rockledge | 157 |
| Ruskin | 152 |
| T | |
| Tallahassee | 144 |
| Tampa | 128 |
| Tampa Airport | 145 |
| Tarpon Springs | 115 |
| Tavares | 79 |
| Titusville | 142 |
| Trenton | 26 |
| Turkey Point | 388 |
| U | |
| Umatilla | 75 |
| V | |
| Venice | 192 |
| Vero Beach | 208 |
| W | |
| Wauchula | 160 |
| Webster | 79 |
| Weeki Wachee | 88 |
| West Palm Beach | 269 |
| Whitney Lab | 78 |
| White Springs | 59 |
| Wildwood | 62 |
| Williston | 21 |
| Winter Garden | 98 |
| Winter Haven | 129 |
| Winter Park | 114 |
| Y | |
| Yankeetown | 54 |
| Z | |
| Zephyrhills | 104 |
| Zolfo Springs | 162 |